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Effect of sub-lethal concentrations of urea on the development of endemic arboreal tree frog, Polypedatus cruciger (Amphibia: Racophoridae)
Ruhuna Journal of Science 14(2): 87–104 · 2024
https://doi.org/10.4038/rjs.v14i2.136
Abstract
Amphibian decline has become a worldwide problem today. Climate change, UV radiation, diseases, contaminants, and fertilizers could be considered as the main reasons. The present study was carried out to investigate the effect of urea on the survival, growth, behaviour and morphology of the endemic arboreal tree frog Polypedatus cruciger (Anura: Ranidae). First, the Lethal Concentration 50 (LC50) of urea on 10 days old post-hatched tadpoles was determined. After the estimation of LC50, the tadpoles were exposed to three sub-lethal concentrations of urea, namely, 7.0 g/L, 7.5 g/L and 8.2 g/L. The effects were investigated from the age of 10 days until they became adults. The impact of sub-lethal concentrations of urea for the survivals, snout-vent length and weight showed significant differences (p
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