Journals › Tropical Agricultural Research
Development of Liquid Culture System for Rapid Multiplication of Gyrinops walla
Tropical Agricultural Research 34(1): 43–51 · 2023
https://doi.org/10.4038/tar.v34i1.8603
Abstract
Agarwood is an expensive resinous compound, produced by Gyrinops walla as defence response to external damages. The tissue culture technique is the best alternative for both rapid multiplication and product synthesis. This study demonstrated an efficient method of rapid multiplication using liquid cultures for G. walla. MS medium supplemented with 1.0 mg/L BAP and 0.1 mg/L IBA, 100 mg/L Myoinositol and 30.0 g/L of sucrose, and 3.0 g/L Phytagel (Sigma-Aldrich) was used as the control (T0). The effect of shoot growth on liquid culture on the same medium was investigated without inert support (T1) and kept on a shaker, with the support of filter paper bridges (T2), rigid support (T3), wet foam (T4), and a double layer of liquid and semi-solid medium (T5). At the end of the sixth week, growth rates and cell doubling times were calculated. Results showed significant differences between treatments at α=0.05 probability level (P
Read at the publisher Citation tracking 🔒
This index records bibliographic metadata and links to the publisher's own page. It does not host or link directly to full text. Citations recorded: 1.